Fluorescence microscopy on bilayers (inverted, high NA>1.2)
Simultaneous optical and electrical readout (low noise, high bandwidth)
MECAopto-inv chips are compatible to a broad range of microscopic objectives.
MECAopto-inv chip with four individually addressable lipid bilayers.
The close-up view of the chip center shows the four micro-electrode-cavities for synchroneous electrical and optical recordings.
Schematic of a single MECAopto-inv cavity with a bilayer for an inverted microscope.
The bilayer can be manually painted over the cavity. The Ag/AgCl electrodes are placed well outside the field of view of the mircroscope for an unimpeded view of the bilayer. The bilayer lies within a working distance of just 220µm of the microscope's objective.
MECAopto-inv chip works with the Orbit mini from Nanion. Thanks to the small footprint of the Orbit Mini it can be placed on an inverted microscop stage to bring the electrical amplifier extremely close to the bilayers for low noise electrical recordings.
Image of Large Unilamelar Vesicles (LUVs) labelled with 1% of Rhodamine B- Phosphatidylethanolamine on a lipid membrane painted on a single MECAopto-inv cavity. λex 560 nm.
The video shows electroporation of a DPhPC-bilayer labelled with 1% of Rhodamine B- Phosphatidylethanolamine on the MECAopto-inv chip. λex 560 nm.